S K I LL S
Bacterial Culture and Maintenance
Bioinformatics (protein structure prediction, docking and analysis)
Biostatistics (SPSS 16, Graphpad, Origin 7.5, Stat-Ease 8.16, Microsoft)
DNA extraction techniques
General Chemistry Lab techniques (calorimetry, chromatography, spectroscopy)
Gene cloning, plasmid isolation, transfection, siRNA designing,
Mammalian Cell Culturing and Maintenance
Microarray (RNA, DNA extraction, sample preparation to data analysis)
Microscopy (conventional, confocal, fluorescence)
PC-1, PC-2, and PC-3 lab work experience
Proteomics (protein extraction and purification, sample preparation to data analysis)
Real Time-PCR & Conventional PCR, Primer Designing
Western blotting (SDS-PAGE / Native Gel Electrophoresis), Immunoprecipitation
h-index = 15, i10-index = 17 (Year 2017)
Google scholar: (https://scholar.google.com.au/citations?user=tat_i-gaaaaj&hl=en
Researchgate Profile: https://www.researchgate.net/profile/waqar_ahmad9
ORCID: orcid.org/0000-0003-0474-6558, Researcher ID: M-3019-2016
Ahmad W, Eber PR: Metformin Attenuates Aβ Pathology Mediated Through Levamisole Sensitive Nicotinic Acetylcholine Receptors in a C. elegans Model of Alzheimer's Disease. Mol Neurobiol 2016
Jahangir Z, Ahmad W*, Shabbiri K. Alternate Phosphorylation/O-GlcNAc Modification on Human Insulin IRSs: A Road towards Impaired Insulin Signaling in Alzheimer and Diabetes. Adv Bioinformatics. 2014;2014:324753
Ahmad W**. Overlapped metabolic and therapeutic links between Alzheimer and Diabetes. Mol Neurobiol 2013, 47: 399-424
Shahid I, Gull S, Ijaz B, Ahmad W, Ansar M, Asad S, Kausar H, Sarwar MT, Khan MK, Hassan S. Stable Huh-7 cell lines expressing hepatitis C virus non-structural proteins of genotype 1a. J Viol Methods 2013
Ahmad W**, Shabbiri K, Adnan A. Exploration of respiratory chain of Nocardia asteroides: purification of Succinate Quinone Oxidoreductase. J Mem Biol 2012
Shabbiri K, Adnan A, Jamil S, Ahmad W, Noor B. Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach. Braz J Microbiol, 2012.
Ahmad W, Ijaz B, Hassan S. Gene expression profiling of HCV genotype 3a initial liver fibrosis and cirrhosis patients using microarray. J Trans Med 2012
Asad S, Ijaz B, Ahmad W, Kausar H, Sarwar MT, Gull S, Shahid I, Khan MK, Hassan S. Development of persistent HCV genotype 3a infection cell culture model in huh-7 cell. Virol J. 2012 Jan 10;9(1):11
Ahmad W**, Shabbiri K, Nazar N, Nazar S, Qaiser S, Mughal MAS. Human linker histones: interplay between phosphorylation and o-β-GlcNAc to mediate chromatin structural modifications. Cell Div 2011, 6:15
Jahan S, Khaliq S, Siddiqi MH, Ijaz B, Ahmad W, Ashfaq UA, Hassan S. Anti-apoptotic effect of HCV core gene of genotype 3a in Huh-7 cell line Virol J. 2011 Nov 23;8:522.
Jahan S, Samreen B, Khaliq S, Ijaz B, Khan M, Siddique MH, Ahmad W, Hassan S. HCV entry receptors as potential targets for siRNA-based inhibition of HCV. Genetic Vaccines and Therapy 2011, 9:15
Kausar H, Gull S, Ijaz B, Ahmad W, Sarwar MT, Iqbal Z, Nawaz Z, Riazuddin S, Hassan S. Huh-7 cell line as an alternative cultural model for the production of human like erythropoietin (EPO). J Trans Med 2011, 9:186
Ijaz B, Ahmad W, Javed FT, Gull S, Sarwar MT, Kausar H, Asad S, Jahan S, Khaliq S, Shahid I, Sumrin A, Hassan S. Association of laboratory parameters with viral factors in patients with hepatitis C. Virol J 2011, 8:361
Jahan S, Samreen B, Khaliq S, Ijaz B, Khan M, Siddique MH, Ahmad W, Hassan S: HCV entry receptors as potential targets for siRNA-based inhibition of HCV. Genet Vaccines Ther 2011, 9:15.
Ahmad W, Ijaz B, Javed FT, Kausar H, Sarwar MT, Gull S, Asad S, Shahid I, Hassan S. HCV genotype specific correlation with serum markers: Higher predictability for Genotype 4a. Virol J 2011, 8:293.
Ahmad W, Shabbiri K, Ijaz B, Asad S, Gull S, Fouzia K, Kausar H, Sarwar MT, Shahid I, Hassan S. Claudin-1 required for HCV virus entry has high potential for phosphorylation and o-glycosylation. Virol J, 2011 8:229.
Ahmad W, Shabbiri K, Ijaz B, Asad S, Nazar N, Nazar S, Fouzia K, Kausar H, Gull S, Tahir Sarwar MT, Shahid I, Hassan S. Serine 204 phosphorylation and O-β-GlcNAC interplay of IGFBP-6 as therapeutic indicator to regulate IGF-II functions in viral mediated hepatocellular carcinoma. Virol J, 2011 8:208.
Ahmad W, Ijaz B, Javed FT, Gull S, Kausar H, Sarwar MT, Asad S, Shahid I, Sumrin A, Khaliq S, Jahan S, Pervaiz A, Hassan S. A comparison of four fibrosis indexes in chronic HCV: Development of new fibrosis-cirrhosis index (FCI). BMC Gastroenterol. 2011,11:44.
Ahmad W, Ijaz B, Gull S, Asad S, Khaliq S, Jahan S, Sarwar MT, Kausar H, Sumrin A, Shahid I, Hassan S. A brief review on molecular, genetic and imaging techniques for HCV fibrosis evaluation. Virol J. 2011 8;8(1):53.
Khaliq S, Jahan S, Ijaz B, Ahmad W, Asad S, Hassan S. Inhibition of HCV 3a genotype by siRNAs targeting envelop genes. Arch Virol, 2011; 156(3):433-442.
Jahan S, Khaliq S, Ijaz B, Ahmad W, Hassan S. Role of HCV Core gene of genotype 1a and 3a and host gene Cox-2 in HCV-induced pathogenesis. Virol J. 2011, 8(1):155.
Ijaz B, Ahmad W*, Javed FT, Gull S, Hassan S. Revised cutoff values of ALT and HBV DNA level can better differentiate HBeAg (-) chronic inactive HBV patients from active carriers. Virol J. 2011 Feb 27;8:86.
Sarwar MT, Kausar H, Ijaz B, Ahmad W, Ansar M, Sumrin A, Ashfaq UA, Gull S, Asad S, Shahid I, Hassan S. NS4A protein as a marker of HCV history suggests that different hepatitis C virus genotypes originally evolved from genotype 1b. Virol J 2011, 8:317.
Jahan S, Khaliq S, Samreen B, Ijaz B, khan m, Ahmad W, Ashfaq AU, Hassan S. Effect of combined siRNA of HCV E2 gene and HCV receptors against HCV. Virol J 2011, 8:295.
Javed FT, Ijaz B, Ahamd W, Jahan S, Khaliq S, Hassan S. Correlation of serum HCV titer, ALP and Bilirubin levels with liver fibrosis stages. IJAVMS, 2010; 4(2): 56-62.
Jahan S, Khaliq S, Afzal N, Mujtiba G, Ahmad W, Ijaz B, Sumrin A, Ashfaq UA, Mahmood S, Hassan S. Regulation of apoptosis by HCV. IJAVMS, 2011, 5(4): 438-443.
Khurshid S, Kashmiri MA, Qureshi Z, Ahmad W. Optimization of glucose oxidase production by Aspergillus niger. Afr J Biotechnol 2011; 10(9): 1674-1678.
Shabbiri K, Ahmad W, Syed Q, Adnan A. Isolation and Purification of Complex II from Proteus mirabilis strain ATCC 29245. Braz J Microbiol, 2010; 41: 796-804.
Ahmad W, Ijaz B, Javed FT, Jahan S, Shahid I, Hassan S. Age specific prevalence and transmission of HCV genotypes in Lahore, Pakistan. World J Gastroenterol 2010; 14: 4321-4328.
Khan M, Jahan S, khaliq S, Ijaz B, Ahmad W, Samreen B, Hassan S. Interaction of the hepatitis C virus (HCV) core with cellular genes in the development of HCV-induced steatosis. Arch Virol, 2010; 155:17251753.
Khaliq S, Jahan S, Ijaz B, Ahmad W, Asad S, Pervaiz A, samreen B, Hassan S. Inhibition of core gene of HCV 3a genotype using synthetic and vector derived siRNAs. Virology J, 2010; 7:318.
Sumrin A, Ahmad W**, Ijaz B, Sarwar MT, Gull S, Kausar H, Jahan S, Asad S, Riazuddin S. Purification and medium optimization of alpha amylase from Bacillus Subtilis 168. Afr J Biotech, 2010; 10(11): 21192129.
Kaleem A, Khan ST, Ahmad W, Saleem M, Khawaja RA, Hussain AS, Shakoori AR and Nasir-ud-Din. Post-Translational Modification of Non-Histone High Mobility Group Nucleosomal Proteins. Pakistan J. Zool., 2007. 39, 57-64.
Kaleem A, Ahmad W, Khan ST, Saleem M, Khawaja RA, Hussain. AS, Shakoori AR, Nasir-ud-Din. In silico, Post-Translational Modification of Histone H1: Regulation of Transcription. Pakistan J. Zool., 2007. 39(2). 87-93.
Ahmad I, Ahmad W, Evelyne-Walker-Nasir, Saleem M, Shakoori AR, Nasir-ud-Din. Histone H1 subtypes in Mouse; interplay between phosphorylation and O-glycosylation”. Pakistan J. Zool., 2007. 40: 34-45.
Ahmad W, Ahmad I, Kaleem A, Evelee-Walker-Nasir, Hoessli DC, Nasir-ud-Din. Human histone H1.5: Role of phosphorylation and glycosylation in cell cycle” Proc. Pakistan Acad. Sci. 2007. 44(2): 85-96.
GENOMIC SEQUENCES SUBMITTED TO NCBI: HM135516; HM135517; HM135518, HM135519, HM135520
Shabbiri K, Ahmad W, Jagadeesan R, Nayak MK, Schirra HJ, Ebert PR. System biology of phosphine controlled insect pests of food commodities. Agricultural Bioscience International Conference, Fargo, North Dakota, USA (Sep 18-21, 2016).
Ahmad W, Ebert P. Dihydrolipoamide dehydrogenase (DLD) as a potential therapeutic target for Alzheimer’s disease. Presented at Genetic Society of Australia Conference, Sydney, Australia (6-9 July 2014).
Ahmad W, Ebert P. dld-1 suppression attenuates the detrimental effects of amyloid beta deposition in a Caenorhabditis elegans model of Alzheimer’s disease. Presented at 3rd Molecular Neurodegeneration Conference, Cannes, France (10-12 Sep, 2013).
Ahmad W, Shakoori RA, Nasir-ud-Din. Prediction of Post-Translational Modification of Histone H1: Regulation of Transcription. Presented at 27th Pakistan Congress of Zoology (International), Feb 27- Mar 1, 2007.
Shabbiri K, Ahmad W, Kashmiri MA, Adnan A, Shafiq-ur-Rehman and Qureshi MH. Isolation and purification of complex II from cell membrane of Proteus mirabilis. Presented at 1st International conference of Chemistry, LCWU, Lahore, 2nd Nov, 2007.
Jahan S, Khaliq S, Ijaz B, Ahmad W, Hassan S. Combined siRNA against HCV Core and COX-2 inhibits genes involved in HCV induced oxidative stress leading to hepatocellualar carcinoma. Presented at 9th Shaukat Khanum Memorial Cancer Symposium “Global Problem Local Solutions”, Lahore, Pakistan, Nov 26th - 28th, 2010.
AWARDS AND ACHIEVEMENTS
• Sponsorship by International Postgraduate Research Scholarships (IPRS for 3 years, Australia 20122015
• Sponsorship by University of Queensland Centennial Scholarship (UQCent) for 3 Years, Australia 20122015
• UQ travel award to attend international conference in Cannes, France, 2013
• Travel grant to attend international conference in Sydney, Australia, 2014
• Won best student award in Biology at college level
CONFERENCES/ SEMINARS/ FORUMS/ GAMES
Oral presentation: Genetic Society of Australia Conference, Sydney, Australia (6-9 July 2014).
Poster presentation: 3rd Molecular Neurodegeneration Conference, Cannes, France (10-12 Sep, 2013).
Oral presentation: 27th Pakistan Congress of Zoology (International), Feb 27- Mar 1, 2007.
First International Conference on the Role of Chemistry & Biochemistry in the National Development, 1618 April 2007, University of Balochistan, Quetta, Pakistan
Proteomics Workshop: For sample preparation to data analysis. Department of Microbiology and Molecular Genetics, University of the Punjab Lahore, Pakistan. 24-25 October 2007.
International Chemistry Conference. “Recent Advances in Chemistry” LCWU, Lahore, 2nd Nov, 2007.
Participation as a resource person at “International training course on microarray technology for gene discovery and expression”. Jointly organized by Islamic Educational, Scientific and Cultural Organization (ISESCO) and Asia Pacific International Molecular Biology Network (A-IMBN) in collaboration with and held at Centre of Excellence in Molecular Biology (CEMB), University of the Punjab Lahore, Pakistan, 0509 October 2009.
Participation as a resource person at “2nd International Training Workshop on DNA Microarray for Gene Expression & Training Workshop on Biosafety in Biomedical Research. Organized by Centre of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore, Pakistan in collaboration Biosafety Association of Pakistan (BSAP), University of Karachi, Karachi, Pakistan, 07-11 March, 2011
1st position in “Biology subject” at Government Degree College Hafiz Abad, 1997-98.
2nd position in “Table Tennis” at Inter-department sports program, 2005-06. GC University Lahore, Pakistan.
English (Read, Write, Speak)
Urdu (Read, Write, Speak)
Punjabi (Read, Write, Speak)
Arabic (Read, Write)